By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)
The use of the time period "advanced" within the identify of this publication is a little ar bitrary and intensely a lot relative with appreciate to time. Many suggestions which have been thought of on the "cutting aspect" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in quite a few laboratories. you will cite freeze-fracture, cryothin sectioning, or certainly many of the box of experiment ning electron microscopy as concrete examples. therefore using the time period "ad vanced options" needs to be interpreted in regards to the current cutting-edge, and turns out to be useful in basic terms in informing the aptitude reader that this quantity isn't really a primer for use as an preliminary creation into easy organic elec tron microscopy. many fantastic volumes have crammed that area of interest long ago few years, and it's not meant that this modest booklet be an entire com pendium of the sphere. additionally, any constrained collection of papers on advanc ed options inevitably displays the personal tastes and arbitrary whims of the editor, thereby with the exception of many both very important systems which the an expert reader will quite simply determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated strategies that have been concept to symbolize complex and but ba sically morphological equipment for gaining elevated ultrastructural informa tion from organic specimens. the current quantity, nonetheless, stresses thoughts which supply particular physicochemical information at the speci mens as well as the structural information.
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Using the time period "advanced" within the identify of this booklet is slightly ar bitrary and extremely a lot relative with appreciate to time. Many options which have been thought of on the "cutting area" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in various laboratories. you can still cite freeze-fracture, cryothin sectioning, or certainly many of the box of experiment ning electron microscopy as concrete examples.
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Additional info for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes
KOEHLER A. Rationale Antibodies labeled with visual markers such as fluorochromes, ferritin, horseradish peroxidase, viruses, hemocyanin, and radioiodine, have recently been used extensively to help understand a variety of biological phenomena (RAFF, 1976; KARNOVSKY and UNANUE, 1973; WAGNER, 1973). In the field of immunology, for example, studies have focused specifically on defining lymphoid populations responsible for elicitation of both cellular and humoral immune responses. To discern these populations, antibodies were prepared against immunologic determinants unique to each cell type.
Scott, R. , Marchesi, V. T: The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles, J. Exp. Med. , Osawa, T, Ukita, T: Purification of galactose-binding phytoagglutinins and phytotoxin by affinity column chromatography using Sepharose. Experientia 28, 84 - 85 (1972) Toms, G. : Phytohaemagglutinins. In: Chemotaxonomy of the Leguminosae. , Turner, B. L. ). New York: Academic Press 1971, pp.
If point-to-point localization is not a consideration, the one-step procedure may then be the method of choice because it requires fewer preparative steps. , 1972 b). However, if receptor site mapping of single cells is being considered, the two-step method may be more appropriate. General procedures for labeling with ferritin and HRP are dealt with in another Chapter of this volume (NICHOLSON, 1977). Therefore, it is the pur- 42 w. D. PERKINS and J. K. KOEHLER pose of this discussion to present methodologies for labeling antibodies with the markers hemocyanin and radioiodine for TEM examination.
Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)